Researchers Database
TOKUDA NOBUKO
Affiliation： Department of Anatomy Position： Professor | |||
Last Updated :2026/04/21
Research achievements
Published Papers
- Newly Found Rat CD103 - Dendritic Cells Represent a Highly Immunogenic Subpopulation of Type-2 Conventional Dendritic Cells, Corresponding to Known Dendritic Cell Subsets in Mice and Humans, Yasushi Sawanobori, Tadayuki Ogawa, Hisashi Ueta, Yusuke Kitazawa, Nobuko Tokuda, Immunology, 174(4), 384-401, 04 Jan. 2025
Original paper
- Selective involution of thymic medulla by cyclosporine A with a decrease of mature thymic epithelia, XCR1+ dendritic cells, and epithelium-free areas containing Foxp3+ thymic regulatory T cells., Sawanobori Yasushi;Kitazawa Yusuke;Ueta Hisashi;Matsuno Kenjiro;Tokuda Nobuko, Histochemistry and cell biology, 16 May 2021
- Suppression of liver transplant rejection by anti-donor MHC antibodies via□ depletion of donor immunogenic dendritic cells., Ueta Hisashi;Xu Xue-Dong;Yu Bin;Kitazawa Yusuke;Yu Enqiao;Hara Yoshiaki;Morita-Nakagawa Miwa;Zhou Shu;Sawanobori Yasushi;Ueha Satoshi;Rokutan Kazuhito;Tanaka Toshiya;Tokuda Nobuko;Matsushima Kouji;Matsuno Kenjiro, International immunology, 01 Dec. 2020
- Monotherapy With Anti-CD70 Antibody Causes Long-Term Mouse Cardiac Allograft Acceptance With Induction of Tolerogenic Dendritic Cells., Zhao Jing;Que Weitao;Du Xiaoxiao;Fujino Masayuki;Ichimaru Naotsugu;Ueta Hisashi;Tokuda Nobuko;Guo Wen-Zhi;Zabrocki Piotr;de Haard Hans;Nonomura Norio;Li Xiao-Kang, Frontiers in immunology, 11, 555996, 2020
- Novel Targeting to XCR1+ Dendritic Cells Using Allogeneic T Cells for Polytopical Antibody Responses in the Lymph Nodes., Kitazawa Yusuke;Ueta Hisashi;Sawanobori Yasushi;Katakai Tomoya;Yoneyama Hiroyuki;Ueha Satoshi;Matsushima Kouji;Tokuda Nobuko;Matsuno Kenjiro, Frontiers in immunology, 10, 1195, 2019
- Optimization of fixative solution for retinal morphology: a comparison with Davidson's fixative and other fixation solutions., Tokuda Kazuhiro;Baron Byron;Kuramitsu Yasuhiro;Kitagawa Takao;Tokuda Nobuko;Morishige Naoyuki;Kobayashi Masaaki;Kimura Kazuhiro;Nakamura Kazuyuki;Sonoda Koh-Hei, Japanese journal of ophthalmology, 62(4), 481-490, Jul. 2018
- The utility of a country-specific Bartonella henselae antigen in an IgM-indirect fluorescent antibody assay for the improved diagnosis of cat scratch disease., Tsuneoka Hidehiro;Yanagihara Masashi;Tanimoto Ayano;Tokuda Nobuko;Otsuyama Ken-Ichiro;Nojima Junzo;Ichihara Kiyoshi, Diagnostic microbiology and infectious disease, 87(1), 22-24, Jan. 2017
- Infection Programs Sustained Lymphoid Stromal Cell Responses and Shapes Lymph Node Remodeling upon Secondary Challenge., Gregory Julia L;Walter Anne;Alexandre Yannick O;Hor Jyh Liang;Liu Ruijie;Ma Joel Z;Devi Sapna;Tokuda Nobuko;Owada Yuji;Mackay Laura K;Smyth Gordon K;Heath William R;Mueller Scott N, Cell reports, 18(2), 406-418, 2017
- Changes in metabolic proteins in ex vivo rat retina during glutamate-induced neural progenitor cell induction., Tokuda Kazuhiro;Kuramitsu Yasuhiro;Baron Byron;Kitagawa Takao;Tokuda Nobuko;Kobayashi Masaaki;Kimura Kazuhiro;Sonoda Koh-Hei;Nakamura Kazuyuki, Molecular and cellular biochemistry, 419(1-2), 177-184, Aug. 2016
- Development of a Highly Specific IgM Enzyme-Linked Immunosorbent Assay for Bartonella henselae Using Refined N-Lauroyl-Sarcosine-Insoluble Proteins for Serodiagnosis of Cat Scratch Disease., Otsuyama Ken-Ichiro;Tsuneoka Hidehiro;Kondou Kaori;Yanagihara Masashi;Tokuda Nobuko;Shirasawa Bungo;Ichihara Kiyoshi, Journal of clinical microbiology, 54(4), 1058-1064, Apr. 2016
- Up-regulation of DRP-3 long isoform during the induction of neural progenitor cells by glutamate treatment in the ex vivo rat retina., Tokuda Kazuhiro;Kuramitsu Yasuhiro;Byron Baron;Kitagawa Takao;Tokuda Nobuko;Kobayashi Daiki;Nagayama Megumi;Araki Norie;Sonoda Koh-Hei;Nakamura Kazuyuki, Biochemical and biophysical research communications, 463(4), 593-599, 07 Aug. 2015
- Fatty Acid Binding Protein 3 Is Involved in n-3 and n-6 PUFA transport in mouse trophoblasts., Islam Ariful;Kagawa Yoshiteru;Sharifi Kazem;Ebrahimi Majid;Miyazaki Hirofumi;Yasumoto Yuki;Kawamura Saki;Yamamoto Yui;Sakaguti Syuiti;Sawada Tomoo;Tokuda Nobuko;Sugino Norihiro;Suzuki Ryoji;Owada Yuji, The Journal of nutrition, 144(10), 1509-1516, Oct. 2014
- Fatty acid binding protein 7 regulates phagocytosis and cytokine production in Kupffer cells during liver injury., Miyazaki Hirofumi;Sawada Tomoo;Kiyohira Miwa;Yu Zhiqian;Nakamura Keiji;Yasumoto Yuki;Kagawa Yoshiteru;Ebrahimi Majid;Islam Ariful;Sharifi Kazem;Kawamura Saki;Kodama Takanori;Yamamoto Yui;Adachi Yasuhiro;Tokuda Nobuko;Terai Shuji;Sakaida Isao;Ishikawa Toshizo;Owada Yuji, The American journal of pathology, 184(9), 2505-2515, Sep. 2014
- Locally existing endothelial cells and pericytes in ovarian stroma, but not bone marrow-derived vascular progenitor cells, play a central role in neovascularization during follicular development in mice., Kizuka-Shibuya Fumie;Tokuda Nobuko;Takagi Kiyoshi;Adachi Yasuhiro;Lee Lifa;Tamura Isao;Maekawa Ryo;Tamura Hiroshi;Suzuki Takashi;Owada Yuji;Sugino Norihiro, Journal of ovarian research, 7, 10, 21 Jan. 2014
- Differential expression and regulatory roles of FABP5 and FABP7 in oligodendrocyte lineage cells., Sharifi Kazem;Ebrahimi Majid;Kagawa Yoshiteru;Islam Ariful;Tuerxun Tuerhong;Yasumoto Yuki;Hara Tomonori;Yamamoto Yui;Miyazaki Hirofumi;Tokuda Nobuko;Yoshikawa Takeo;Owada Yuji, Cell and tissue research, 354(3), 683-695, Dec. 2013
- Fatty acid binding protein 7 as a marker of glioma stem cells., Morihiro Yusuke;Yasumoto Yuki;Vaidyan Linda Koshy;Sadahiro Hirokazu;Uchida Tomoyuki;Inamura Akinori;Sharifi Kazem;Ideguchi Makoto;Nomura Sadahiro;Tokuda Nobuko;Kashiwabara Shoji;Ishii Aya;Ikeda Eiji;Owada Yuji;Suzuki Michiyasu, Pathology international, 63(11), 546-553, Nov. 2013
- Cooling treatment transiently increases the permeability of brain capillary endothelial cells through translocation of claudin-5., Inamura Akinori;Adachi Yasuhiro;Inoue Takao;He Yeting;Tokuda Nobuko;Nawata Takashi;Shirao Satoshi;Nomura Sadahiro;Fujii Masami;Ikeda Eiji;Owada Yuji;Suzuki Michiyasu, Neurochemical research, 38(8), 1641-1647, Aug. 2013
- Fatty acid-binding protein 4 (FABP4) and FABP5 modulate cytokine production in the mouse thymic epithelial cells., Adachi Yasuhiro;Hiramatsu Sumie;Tokuda Nobuko;Sharifi Kazem;Ebrahimi Majid;Islam Ariful;Kagawa Yoshiteru;Koshy Vaidyan Linda;Sawada Tomoo;Hamano Kimikazu;Owada Yuji, Histochemistry and cell biology, 138(3), 397-406, Sep. 2012
- Involvement of bone marrow-derived vascular progenitor cells in neovascularization during formation of the corpus luteum in mice., Kizuka Fumie;Tokuda Nobuko;Takagi Kiyoshi;Adachi Yasuhiro;Lee Lifa;Tamura Isao;Maekawa Ryo;Taketani Toshiaki;Tamura Hiroshi;Suzuki Takashi;Owada Yuji;Sugino Norihiro, Biology of reproduction, 87(3), 55, Sep. 2012
- Reduced size of sebaceous gland and altered sebum lipid composition in mice lacking fatty acid binding protein 5 gene., Sugawara Tomoko;Nemoto Kei;Adachi Yasuhiro;Yamano Nozomi;Tokuda Nobuko;Muto Masahiko;Okuyama Ryuhei;Sakai Shingo;Owada Yuji, Experimental dermatology, 21(7), 543-546, Jul. 2012
- Cutaneous magnetic stimulation reduces rat chronic pain via activation of the supra-spinal descending pathway., Shiiba Shun-ji;Yamamoto Satoru;Sasaki Hironori;Nishi Mitsuharu;Ishikawa Kozo;Yasuda Seiko;Tokuda Nobuko;Nakanishi Osamu;Ishikawa Toshizo, Cellular and molecular neurobiology, 32(2), 245-253, Mar. 2012
- FABP7 expression in normal and stab-injured brain cortex and its role in astrocyte proliferation., Sharifi Kazem;Morihiro Yusuke;Maekawa Motoko;Yasumoto Yuki;Hoshi Hisae;Adachi Yasuhiro;Sawada Tomoo;Tokuda Nobuko;Kondo Hisatake;Yoshikawa Takeo;Suzuki Michiyasu;Owada Yuji, Histochemistry and cell biology, 136(5), 501-513, Nov. 2011
- Fatty acid-binding protein regulates LPS-induced TNF-alpha production in mast cells., Yamamoto Noriko;Kaneko Izumi;Motohashi Keiju;Sakagami Hiroyuki;Adachi Yasuhiro;Tokuda Nobuko;Sawada Tomoo;Furukawa Hiroshi;Ueyama Yoshiya;Fukunaga Kohji;Ono Masao;Kondo Hisatake;Owada Yuji, Prostaglandins, leukotrienes, and essential fatty acids, 79(1-2), 21-26, 03 Aug. 2008
- Identification and characterization of major histocompatibility complex class II compartments in cortical thymic epithelial cells., Arudchelvan Yamini;Nishimura Yukio;Tokuda Nobuko;Sawada Tomoo;Ueyama Yoshiya;Fukumoto Tetsuo, The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology, 274(1), 798-806, Sep. 2003
- Differential expression of MHC class II antigens and cathepsin L by subtypes of cortical epithelial cells in the rat thymus: an immunoelectron microscopic study., Arudchelvan Yamini;Nishimura Yukio;Tokuda Nobuko;Sawada Tomoo;Shinozaki Fumihiko;Fukumoto Tetsuo, Journal of electron microscopy, 51(3), 173-181, 2002
Unclear
Books etc
- 10 Apr. 2024, Textbook, 978-4-910513-20-1
- Joint work, 20 Nov. 2023, Textbook
- Supervisor, 16 Nov. 2023, Dictionary or encycropedia, 978-4-05-205699-4
- Joint work, 15 Jan. 2023, 608, 464-487, Textbook, 978-4-8404-7831-1
Presentations
- Rat CD103-MHCII+CD45R-CD161+CD172a+ cells are a novel potent subpopulation of cDC2, corresponding to known mouse and human subpopulations., Yasushi Sawanobori, Tadayuki Ogawa, Hisashi Ueta, Yusuke Kitazawa, Nobuko Tokuda, APPW2025, 19 Mar. 2025, 17 Mar. 2025, 19 Mar. 2025, Oral presentation, Chiba, Japan, Japan, Dendritic cells (DCs) are known for their potent capabilities in antigen presentation, T cell priming, and cytokine secretion. They consist of XCR1+ conventional DC1 (cDC1), CD172a+ cDC2, and plasmacytoid DC subsets. While CD11c serves as the primary marker for DCs in mice and humans, rat DCs have traditionally been identified by CD103 molecules. However, this history cannot exclude the possibility of CD103- DC existence in rats. To investigate this possibility, we examined low-density cells from the rat spleen and MHCII-enriched rat thymic cells, finding CD103-MHCII+CD45R-CD161+CD172a+ cells. These cells were negative for CD103 and the B cell marker CD45R, but positive for another rat DC marker CD161 and the cDC2 marker CD172a. For further characterization, we performed microarray analyses on these cells. Principal component analysis, marker gene expression, and similarity to murine counterparts indicated that these cells represent a subpopulation of cDC2. Consequently, we designated this novel subset and the classic rat cDC2 as CD103- cDC2 and CD103+ cDC2, respectively. Subsequently, we investigated the functionality of the rat CD103- and CD103+ cDC2 subsets. Gene set enrichment analyses suggested that the rat CD103- cDC2 subsets are enriched for immune-associated terms, while the rat CD103+ cDC2 subsets are enriched for cell cycle-associated terms. Consistent with this prediction, the rat CD103- cDC2 cells induced enhanced T cell proliferation in mixed lymphocyte reaction assays. Given that mouse and human cDC2 subsets include subpopulations such as cDC2a, cDC2b, inf-cDC2, and moDC, we compared the rat CD103- and CD103+ cDC2 subsets to these subpopulations. The rat CD103- cDC2s expressed markers associated with cDC2a, cDC2b, inf-cDC2, and moDC, whereas the rat CD103+ cDC2s expressed exclusively cDC2a markers. These findings suggest that a substantial and potent population of DCs may have been overlooked in studies relying on anti-CD103 criteria. Researchers working with rat models should consider the presence of CD103- cDC2s in their investigations., False, Domestic journal
- A single-cell analysis revealed tissue-restricted antigen-expressing fibroblasts accumulated in epithelium-free areas in rat thymic medulla, Yasushi Sawanobori, Hisashi Ueta, Yusuke Kitazawa, and Nobuko Tokuda, The 53rd Annual Meeting of the Japanese Society for Immunology, 03 Dec. 2024, 03 Dec. 2024, 05 Dec. 2024, Oral presentation, Nagasaki, Japan, Japan, Several reports have indicated that not only medullary thymic epithelial cells (mTECs) but also thymic medullary fibroblasts (mFbs) express tissue-restricted antigens (TRAs) that are essential for the negative selection (Nitta 2020, Nitta 2021). On the other hand, we have reported that there are medullary epithelium-free areas (mEFAs) that lack mTECs in the medulla of rat thymus (Sawanobori 2014, Sawanobori 2021). We recently performed immunohistochemistry upon some candidate antigens to reveal accumulated cells in the mEFAs, finding the accumulation of PDGFRb+ fibroblasts in this area. Based on these facts, we performed a single-cell RNA-seq (scRNA-seq) analysis on rat thymic stromal cells to investigate whether the rat mEFAs are the place to present TRAs expressed by mFbs. The umap clustering revealed five fibroblast subsets: Fb1-4s and capsular fibroblasts (capFbs). Fb1s and Fb2s expressed Pdgfra mainly. Fb3s and Fb4s expressed Pdgfrb and significant TRA genes, suggesting that they are the mFbs accumulated in the rat mEFAs. The velocity analysis revealed that Fb1s are an independent cluster, Fb2s are progenitors of capFbs, and Fb3s are the progenitors of Fb4s. Our research has demonstrated that TRA-expressing PDGFRb+ Fbs accumulate in the rat mEFAs, suggesting that mEFAs are where Fb-derived TRA are presented. Moreover, we have discovered novel fibroblast subsets: Pdgfra+ Fb1s and capFb progenitor Fb2s. Now, investigations into functions and localizations of these novel subsets are ongoing. An additional remark: The scRNA-seq also revealed subpopulations of rat mTECs that have been poorly understood. Data concerning mTECs will also be presented., False, Domestic journal